The sequence of reagents used in Gram staining is () A: Safranin solution → iodine solution → alcohol →<br/>crystal violet B: crystal violet → alcohol → iodine solution → Safranin<br/>solution C: Crystal violet → iodine solution → alcohol → Safranin<br/>solution D: Safranin solution → alcohol → crystal violet → iodine<br/>solution E: Safranin solution → crystal violet → iodine solution →<br/>alcohol

The following substances are used in Gram staining except A: iodine B: crystal violet C: alcohol D: safranin E: congo red

Which of the following staining solution are used in the bacterial Gram staining method？ ( ) A: Crystal violet B: Iodine solution C: Ethanol D: Safranin

Gram staining involves the following steps like: ( ) A: Make a slide of bacteria sample to be stained. B: Add the secondary stain safranin to the slide and incubate for 1 minute. C: Rinse sample or slide with acetone or alcohol for 30 seconds and rinse with a gentle stream of water. D: Add the primary stain (crystal violet) to the sample or slide and incubate for 1 minute.